Increasing the coverage of the N-terminome with LysN amino terminal enrichment (LATE)

Rawad Hanna, Andrey Rozenberg, Tali Lavy, Oded Kleifeld

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

The field of N-terminomics has been advancing with the development of novel methods that provide a comprehensive and unbiased view of the N-terminome. Negative selection N-terminomics enables the identification of free and naturally modified protein N-termini. Here, we present a streamlined protocol that combines two negative selection N-terminomics methods, LATE and HYTANE, to increase N-terminome coverage by 1.5-fold compared to using a single methodology. Our protocol includes sample preparation and data analysis of both methods and can be applied to studying the N-terminome of diverse samples. The suggested approach enables researchers to achieve a more detailed and accurate understanding of the N-terminome.

Original languageEnglish
Title of host publicationModifications and targeting of protein termini
Subtitle of host publicationPart B
EditorsThomas Arnesen
Pages1-28
Number of pages28
DOIs
StatePublished - Jan 2023

Publication series

NameMethods in Enzymology
Volume686
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • HYTANE
  • LATE
  • LysN
  • N-terminomics
  • Peptidyl-Lys metalloendopeptidase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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