Abstract
The present invention provides methods and systems for enhanced production and/or secretion of extracellular vesicles (EVs) from muscle cells utilizing various dynamic mechanical loading profiles thereon, cultured on three-dimensional (3D) scaffolds. The scaffolds may comprise a plurality of layers, wherein each layer comprises a plurality of elastic microfibers, and wherein the microfibers are aligned in parallel to a longitudinal axis and to each other. The elastic 3D scaffold may be configured to undergo dynamic mechanical loading profiles and support an expansion of a population of muscle cells cultured thereon into a 3D multi-layer structure of muscle cells, wherein said 3D multi-layer structure is configured to produce and/or secret extracellular vesicles into a medium.
| Original language | American English |
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| Patent number | US2023287350 |
| IPC | C12N 5/ 077 A I |
| Priority date | 16/08/21 |
| State | Published - 14 Sep 2023 |