TY - JOUR
T1 - Single-allele analysis of transcription kinetics in living mammalian cells
AU - Yunger, Sharon
AU - Rosenfeld, Liat
AU - Garini, Yuval
AU - Shav-Tal, Yaron
N1 - Funding Information:
We thank R. Pestell (Thomas Jefferson University) for the cyclin D1 promoter, C. Cardoso (Technische Universität Darmstadt) for RFP-PCNA and R. Drummer (Bar-Ilan University) for statistical analysis. This work was supported by grants to Y.S.-T. by the Israel Cancer Research Fund (Research Career Development Award), the Israel Ministry of Health, the Israel Cancer Association, the Alon Fellowship and the Jane Stern Lebell Family Fellowship of Bar-Ilan University; and to Y.S.-T. and Y.G. by the Israel Science Foundation Bikura grant. We thank the Israel Science Foundation for the fluorescence live-cell imaging microscopes.
PY - 2010/8
Y1 - 2010/8
N2 - We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
AB - We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
UR - http://www.scopus.com/inward/record.url?scp=77955174763&partnerID=8YFLogxK
U2 - 10.1038/nmeth.1482
DO - 10.1038/nmeth.1482
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 20639867
AN - SCOPUS:77955174763
SN - 1548-7091
VL - 7
SP - 631
EP - 633
JO - Nature Methods
JF - Nature Methods
IS - 8
ER -