Single-particle tracking for studying the dynamic properties of genomic regions in live cells

Irena Bronshtein Berger; Eldad Kepten; Yuval Garini

doi:10.1007/978-1-62703-526-2_10

Single-particle tracking for studying the dynamic properties of genomic regions in live cells

Irena Bronshtein Berger, Eldad Kepten, Yuval Garini

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The appropriate functioning of living cells depends on a variety of dynamic processes that necessitate delicate motion, transportation, association, and disassociation in time and space. Different dynamic patterns such as directed motion, normal diffusion, and restricted diffusion take part at different length scales, and their identification serves as a tool for exploring biochemical processes. Here we describe single-particle tracking which is a powerful method that allows the characterization of dynamic processes on the single-molecule or single-particle level with nanometer spatial and sub-second temporal precision. In particular, we describe the cell preparation procedures, microscopy imaging, and image analysis processes for following telomere dynamics in living mammalian cells.

Original language	English
Title of host publication	Imaging Gene Expression
Subtitle of host publication	Methods and Protocols
Pages	139-151
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-62703-526-2_10
State	Published - 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1042
ISSN (Print)	1064-3745

Keywords

Chromatin
Diffusion
Fluorescence microscopy
Nuclear organization
Single-particle tracing

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-62703-526-2_10

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KW - Diffusion

KW - Fluorescence microscopy

KW - Nuclear organization

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Single-particle tracking for studying the dynamic properties of genomic regions in live cells

Abstract

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Keywords

ASJC Scopus subject areas

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