Sodium dependency of the inward potassium rectifier in horizontal cells isolated from the white bass retina

Cindy L. Pfeiffer-Linn; Ido Perlman; Eric M. Lasater

doi:10.1016/0006-8993(95)00964-4

Sodium dependency of the inward potassium rectifier in horizontal cells isolated from the white bass retina

Cindy L. Pfeiffer-Linn, Ido Perlman, Eric M. Lasater

Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The ionic properties underlying the inwardly rectifying potassium current in cultured voltage-clamped white bass horizontal cells were studied. Anomalous rectification was apparent upon membrane hype rpolarization with a reversal potential depolarized from the predicted value of E_K In raised extracellular potassium, the current increased and the reversal potential shifted toward a more depolarized membrane potential. Solutions containing decreased sodium caused a rapid decrease in the inward rectifier current but only slightly affected the reversal potential. Extracellular cesium or barium caused a reversible voltage-dependent reduction of the inward current. We interpret these results to mean that the inward rectifying channel in white bass horizontal cells is mainly permeable to potassium ions, but is sodium dependent. It may shape the photoresponses of the horizontal cells and may contribute to a hyperpolarization activated conductance increase measured in situ.

Original language	English
Pages (from-to)	81-88
Number of pages	8
Journal	Brain Research
Volume	701
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(95)00964-4
State	Published - 1 Dec 1995

Keywords

Horizontal cell
Inward rectifier
Potassium current
Retina
Sodium dependency
Voltage-clamp

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0006-8993(95)00964-4

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title = "Sodium dependency of the inward potassium rectifier in horizontal cells isolated from the white bass retina",

abstract = "The ionic properties underlying the inwardly rectifying potassium current in cultured voltage-clamped white bass horizontal cells were studied. Anomalous rectification was apparent upon membrane hype rpolarization with a reversal potential depolarized from the predicted value of EK In raised extracellular potassium, the current increased and the reversal potential shifted toward a more depolarized membrane potential. Solutions containing decreased sodium caused a rapid decrease in the inward rectifier current but only slightly affected the reversal potential. Extracellular cesium or barium caused a reversible voltage-dependent reduction of the inward current. We interpret these results to mean that the inward rectifying channel in white bass horizontal cells is mainly permeable to potassium ions, but is sodium dependent. It may shape the photoresponses of the horizontal cells and may contribute to a hyperpolarization activated conductance increase measured in situ.",

keywords = "Horizontal cell, Inward rectifier, Potassium current, Retina, Sodium dependency, Voltage-clamp",

author = "Pfeiffer-Linn, {Cindy L.} and Ido Perlman and Lasater, {Eric M.}",

note = "Funding Information: We would like to thank Drs. Eduardo Solessio and D. Linn for helpful discussion throughout the course of this study. The work was supported by EY05972 to E.M. Lasater from the National Eye Institute and from an unrestricted grant to the Department of Ophthalmology from Research to Prevent Blindness (R.P.B.). E.M.L. is an RPB William and Mary Greve International Research Scholar.",

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AU - Pfeiffer-Linn, Cindy L.

AU - Perlman, Ido

AU - Lasater, Eric M.

N1 - Funding Information: We would like to thank Drs. Eduardo Solessio and D. Linn for helpful discussion throughout the course of this study. The work was supported by EY05972 to E.M. Lasater from the National Eye Institute and from an unrestricted grant to the Department of Ophthalmology from Research to Prevent Blindness (R.P.B.). E.M.L. is an RPB William and Mary Greve International Research Scholar.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - The ionic properties underlying the inwardly rectifying potassium current in cultured voltage-clamped white bass horizontal cells were studied. Anomalous rectification was apparent upon membrane hype rpolarization with a reversal potential depolarized from the predicted value of EK In raised extracellular potassium, the current increased and the reversal potential shifted toward a more depolarized membrane potential. Solutions containing decreased sodium caused a rapid decrease in the inward rectifier current but only slightly affected the reversal potential. Extracellular cesium or barium caused a reversible voltage-dependent reduction of the inward current. We interpret these results to mean that the inward rectifying channel in white bass horizontal cells is mainly permeable to potassium ions, but is sodium dependent. It may shape the photoresponses of the horizontal cells and may contribute to a hyperpolarization activated conductance increase measured in situ.

AB - The ionic properties underlying the inwardly rectifying potassium current in cultured voltage-clamped white bass horizontal cells were studied. Anomalous rectification was apparent upon membrane hype rpolarization with a reversal potential depolarized from the predicted value of EK In raised extracellular potassium, the current increased and the reversal potential shifted toward a more depolarized membrane potential. Solutions containing decreased sodium caused a rapid decrease in the inward rectifier current but only slightly affected the reversal potential. Extracellular cesium or barium caused a reversible voltage-dependent reduction of the inward current. We interpret these results to mean that the inward rectifying channel in white bass horizontal cells is mainly permeable to potassium ions, but is sodium dependent. It may shape the photoresponses of the horizontal cells and may contribute to a hyperpolarization activated conductance increase measured in situ.

KW - Horizontal cell

KW - Inward rectifier

KW - Potassium current

KW - Retina

KW - Sodium dependency

KW - Voltage-clamp

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Sodium dependency of the inward potassium rectifier in horizontal cells isolated from the white bass retina

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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