TY - JOUR
T1 - Silica Nanoparticles for Intracellular Protein Delivery
T2 - a Novel Synthesis Approach Using Green Fluorescent Protein
AU - Schmidt, Sarah
AU - Tavernaro, Isabella
AU - Cavelius, Christian
AU - Weber, Eva
AU - Kümper, Alexander
AU - Schmitz, Carmen
AU - Fleddermann, Jana
AU - Kraegeloh, Annette
N1 - Publisher Copyright:
© 2017, The Author(s).
PY - 2017
Y1 - 2017
N2 - In this study, a novel approach for preparation of green fluorescent protein (GFP)-doped silica nanoparticles with a narrow size distribution is presented. GFP was chosen as a model protein due to its autofluorescence. Protein-doped nanoparticles have a high application potential in the field of intracellular protein delivery. In addition, fluorescently labelled particles can be used for bioimaging. The size of these protein-doped nanoparticles was adjusted from 15 to 35 nm using a multistep synthesis process, comprising the particle core synthesis followed by shell regrowth steps. GFP was selectively incorporated into the silica matrix of either the core or the shell or both by a one-pot reaction. The obtained nanoparticles were characterised by determination of particle size, hydrodynamic diameter, ζ-potential, fluorescence and quantum yield. The measurements showed that the fluorescence of GFP was maintained during particle synthesis. Cellular uptake experiments demonstrated that the GFP-doped nanoparticles can be used as stable and effective fluorescent probes. The study reveals the potential of the chosen approach for incorporation of functional biological macromolecules into silica nanoparticles, which opens novel application fields like intracellular protein delivery.
AB - In this study, a novel approach for preparation of green fluorescent protein (GFP)-doped silica nanoparticles with a narrow size distribution is presented. GFP was chosen as a model protein due to its autofluorescence. Protein-doped nanoparticles have a high application potential in the field of intracellular protein delivery. In addition, fluorescently labelled particles can be used for bioimaging. The size of these protein-doped nanoparticles was adjusted from 15 to 35 nm using a multistep synthesis process, comprising the particle core synthesis followed by shell regrowth steps. GFP was selectively incorporated into the silica matrix of either the core or the shell or both by a one-pot reaction. The obtained nanoparticles were characterised by determination of particle size, hydrodynamic diameter, ζ-potential, fluorescence and quantum yield. The measurements showed that the fluorescence of GFP was maintained during particle synthesis. Cellular uptake experiments demonstrated that the GFP-doped nanoparticles can be used as stable and effective fluorescent probes. The study reveals the potential of the chosen approach for incorporation of functional biological macromolecules into silica nanoparticles, which opens novel application fields like intracellular protein delivery.
KW - Bioimaging
KW - Core-shell silica nanoparticles
KW - Green fluorescent protein
KW - Protein delivery
UR - http://www.scopus.com/inward/record.url?scp=85029831697&partnerID=8YFLogxK
U2 - 10.1186/s11671-017-2280-9
DO - 10.1186/s11671-017-2280-9
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AN - SCOPUS:85029831697
SN - 1931-7573
VL - 12
JO - Nanoscale Research Letters
JF - Nanoscale Research Letters
M1 - 545
ER -